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PubMed ID 27526320 Pubmed 日付 2016/Aug/15
タイトル Genetic variation in ZmVPP1 contributes to drought tolerance in maize seedlings.
ジャーナル Nature genetics  2016/Aug/15  ()   
著者 Wang Xianglan X,Wang Hongwei H,Liu Shengxue S,Ferjani Ali A,Li Jiansheng J,Yan Jianbing J,Yang Xiaohong X,Qin Feng F
抄録 Maize production is threatened by drought stress worldwide. Identification of the genetic components underlying drought tolerance in maize is of great importance. Here we report a genome-wide association study (GWAS) of maize drought tolerance at the seedling stage that identified 83 genetic variants, which were resolved to 42 candidate genes. The peak GWAS signal showed that the natural variation in ZmVPP1, encoding a vacuolar-type H(+) pyrophosphatase, contributes most significantly to the trait. Further analysis showed that a 366-bp insertion in the promoter, containing three MYB cis elements, confers drought-inducible expression of ZmVPP1 in drought-tolerant genotypes. Transgenic maize with enhanced ZmVPP1 expression exhibits improved drought tolerance that is most likely due to enhanced photosynthetic efficiency and root development. Taken together, this information provides important genetic insights into the natural variation of maize drought tolerance. The identified loci or genes can serve as direct targets for both genetic engineering and selection for maize trait improvement.

 ZmVPP1 in drought tolerance

東京学芸大学自然科学系生命科学分野  Ferjani Ali研究室 Ferjani Ali  投稿日時[2016-08-18 22:13:34]

Maize is a major crop grown for food, feed and fuel, however its production is threatened by drought stress worldwide. Thus, there is tremendous interest in and demand for improving maize drought tolerance through biotechnology. Identification of the genetic components underlying drought tolerance in maize is of great importance.

A genome-wide association study (GWAS) of maize drought tolerance at the seedling stage that identified 83 genetic variations, which were resolved to 42 candidate genes. The peak GWAS signal showed that the natural variation in ZmVPP1, encoding a vacuolar-type H+-pyrophosphatase, contributes most significantly to the trait. Further analysis showed that a 366-bp insertion in the promoter (InDel-379), containing three MYB cis-elements, confers drought inducible expression of ZmVPP1 in drought-tolerant genotypes. Introgression the tolerant allele of ZmVPP1 into a drought-sensitive genetic background greatly improved plant drought tolerance. Transgenic maize with enhanced ZmVPP1 expression exhibits improved drought tolerance that is most likely due to enhanced photosynthetic efficiency and root development. Taken together, this information provides important genetic insights into the natural variation of maize drought tolerance. The identified loci and genes can serve as direct targets for both genetic engineering and selection for maize trait improvement.