文部省 科学研究費補助金 新学術領域研究 植物発生ロジックの多元的開拓

PubMed ID 28556940 Pubmed 日付 2017/May/30
タイトル Three TOB1-related YABBY genes are required to maintain proper function of the spikelet and branch meristems in rice.
ジャーナル The New phytologist  2017/May/30  ()   
著者 Tanaka Wakana W,Toriba Taiyo T,Hirano Hiro-Yuki HY
抄録 YABBY genes play important roles in the development of lateral organs such as leaves and floral organs in Angiosperms. However, the function of YABBY genes is poorly understood in monocots. We focused on three rice (Oryza sativa) YABBY genes, TONGARI-BOUSHI (TOB1, TOB2, TOB3), which are closely related to Arabidopsis (Arabidopsis thaliana) FILAMENTOUS FLOWER (FIL). To elucidate the function of these YABBY genes, we employed a reverse genetic approach. TOB genes were expressed in bract and lateral organ primordia, but not in meristems. RNAi knockdown of TOB2 or TOB3 in the tob1 mutant caused abnormal spikelet development. Furthermore, simultaneous knockdown of both TOB2 and TOB3 in tob1 affected not only spikelet, but also inflorescence development. In severe cases, the inflorescences comprised naked branches without spikelets. Analysis of inflorescence development at an early stage showed that the observed phenotypic defects were closely associated with a failure to initiate and maintain reproductive meristems. These results indicate that the TOB genes regulate the maintenance and fate of all reproductive meristems. It is likely that the function of FIL/TOB clade YABBY genes has been conserved between Arabidopsis and rice to maintain the proper function of meristems, even though these genes are expressed in lateral organ primordia.

 YABBY遺伝子によるメリステム維持

東京大学 進化遺伝学研究室 平野博之  投稿日時[2017-06-06 16:41:18]

TOB1/2/3の3つのYABBY遺伝子は側生器官で発現し,細胞非自律的にメリステムの活性を維持し,花や花序の発生に重要な役割を果たしています.tob1変異体では花の発生が異常となりますが,tob1変異体でTOB2とTOB3の発現を抑制すると,花自体の形成が阻害されてされてしまいます.